– Calculate CFU from this figure according to the formula below: CFU Number of Colonies × Dilution Factor x Volume Of Sample Added To Plate / 100 mL. Example: If you see 2 colonies, then 10µL was added. So multiply 2×1020. 20÷100ml0.02 so 0.02cfu /ml is obtained. Similarly if you get 5 colonies then divide 5by100.05cfe/.01 ml. Miles & Misra Method ...

When doing dilution problems, remember the following:Note that using this method, the answer in CFUs per one milliliter or per one gram is derived. ...Use only those plates with colony counts between 30 and 300. ...Note that all individual dilutions and the amount plated are multiplied together.The initial dilution is often different from the subsequent dilutions. ... More items... ...

Serial Dilution- Definition, Formula, Calculator, Procedure, UsesSerial Dilution Definition. ...Objectives of Serial Dilution. ...Serial Dilution formula/calculations. ...Online Serial dilution calculator. ...Procedure of Serial Dilution. ...Applications/Uses. ...Limitation/Problems. ...Examples. ...References. ...Sources ...

Key considerations when making solutions:Make sure to always research the precautions to use when working with specific chemicals.Be sure you are using the right form of the chemical for the calculations. ...Always use a graduate cylinder to measure out the amount of water for a solution, use the smallest size of graduated cylinder that will accommodate the entire solution. ... More items... ...